About

Here we present an online tool that predicts the proteome-wide all possible pairs of three transitions that are unique or specific to the given peptide. "Digest", a web based tool, offers a novel alternative for rapid design of MRM transitions for proteomics research. Digest has been developed to predict all the possible fragments that can be produced in collision cell of mass spectrometry; from peptide of multiple enzymatically (trypsin, trypsinP, Glu C and ….) digested protein. This includes all the b, y, a and other ions with some frequently occurring fixed and variable post translational modifications (PTMs). Additionally, the program uses combination of knowledge of fundamental rules for design of optimal MRM transitions and its algorithm has a quality assurance from different databases (Uniprot etc) that the designed MRM transitions for the peptide of interest is unique for a given protein. Apart from human proteome database, Digest can be use to design MRM assays for multiple frequently used model organism including PTMs. Additionally, Digest has feature that can be use to find interfering transition, if any, for cross-contamination (other organism).